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1Department of Physics,Pohang University of Science and Technology (POSTECH), 2School of Biological Sciences,Seoul National University, 3Institute for Molecular Biology and Genetics,Seoul National University, 4School of Interdisciplinary Bioscience and Bioengineering,Pohang University of Science and Technology (POSTECH)
Chapters
- 00:04Introduction
- 00:59Apparatus Construction
- 02:36Flow Cell Fabrication
- 03:50Assembly of Bead‐Tether Constructs
- 04:20Identification of Target Constructs
- 05:00Calibration of Magnetic Force
- 05:50Bead Tracking for Extension Measurement
- 06:28Results: Effect of Magnetic Force on DNA Constructs
- 08:11Conclusion
Hier beschrijven we een snelle magnetische pincetopstelling die nanomechanische metingen uitvoert op krachtgevoelige biomoleculen met de maximale snelheid van 1,2 kHz. We introduceren de toepassing ervan op DNA-haarspelden en SNARE-complexen als modelsystemen, maar het zal ook van toepassing zijn op andere moleculen die betrokken zijn bij mechanobiologische gebeurtenissen.
Tags
Magnetic TweezersNanomechanical MeasurementsForce-sensitive ElementsBiomoleculesStructural ChangesNucleic AcidsProteinsMechanosensitive ProteinsCardiovascular DisordersMusculoskeletal DisordersHigh-speed CMOS CameraMotorized Linear StageRotary Stepper MotorSuper Luminescent DiodePiezo Lens ScannerCoverslip PreparationPegylation
