/
/
High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1,515 Views
•
08:50 min
•
May 12, 2023
DOI:
1Department of Physics,Pohang University of Science and Technology (POSTECH), 2School of Biological Sciences,Seoul National University, 3Institute for Molecular Biology and Genetics,Seoul National University, 4School of Interdisciplinary Bioscience and Bioengineering,Pohang University of Science and Technology (POSTECH)
Summary
Automatically generated
Here, we describe a high-speed magnetic tweezer setup that performs nanomechanical measurements on force-sensitive biomolecules at the maximum rate of 1.2 kHz. We introduce its application to DNA hairpins and SNARE complexes as model systems, but it will be also applicable to other molecules involved in mechanobiological events.
Explore More Videos
Magnetic TweezersNanomechanical MeasurementsForce-sensitive ElementsBiomoleculesStructural ChangesNucleic AcidsProteinsMechanosensitive ProteinsCardiovascular DisordersMusculoskeletal DisordersHigh-speed CMOS CameraMotorized Linear StageRotary Stepper MotorSuper Luminescent DiodePiezo Lens ScannerCoverslip PreparationPegylation
Read Article
Cite this Article
Park, C., Yang, T., Rah, S., Kim, H. G., Yoon, T., Shon, M. J. High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements. J. Vis. Exp. (195), e65137, doi:10.3791/65137 (2023).
Copy