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Isolatie van extracellulaire blaasjes met een laag endotoxinegehalte afgeleid van kankercellijnen
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JoVE 신문 면역학 및 감염병학
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
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Isolatie van extracellulaire blaasjes met een laag endotoxinegehalte afgeleid van kankercellijnen

799 Views

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06:28 min

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February 17, 2023

DOI:

10.3791/65062-v

DOI:
10.3791/65062-v

•

06:28 min
February 17, 2023

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4 Views
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1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Summary

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Het voorgestelde protocol bevat richtlijnen over hoe besmetting met endotoxine kan worden voorkomen tijdens de isolatie van extracellulaire blaasjes uit celkweeksupernatanten en hoe deze op de juiste manier kunnen worden geëvalueerd.

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Cite this Article

Babula, A., Siemińska, I., Baj-Krzyworzeka, M. Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines. J. Vis. Exp. (192), e65062, doi:10.3791/65062 (2023).

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