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Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

DOI:
10.3791/65062-v

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06:28 min

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February 17, 2023

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, ,
1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Capitoli

  • 00:04Introduction
  • 00:42Cell Culture
  • 01:44Isolation of Extracellular Vesicles
  • 03:14Western Blotting
  • 04:47Results: Characterization of Extracellular Vesicles
  • 05:35Conclusion

Summary

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Traduzione automatica

The proposed protocol includes guidelines on how to avoid contamination with endotoxin during the isolation of extracellular vesicles from cell culture supernatants, and how to properly evaluate them.

Tags

Extracellular VesiclesEndotoxinLow-endotoxinCell CultureSupernatantUltracentrifugationFiltrationSW480SW620PBSFBSAseptic ProcedurePyrogenicLow Protein Binding

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