Journal
/
免疫与感染
/
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

DOI:
10.3791/65062-v

•

06:28 min

•

February 17, 2023

•

, ,
1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Chapters

  • 00:04Introduction
  • 00:42Cell Culture
  • 01:44Isolation of Extracellular Vesicles
  • 03:14Western Blotting
  • 04:47Results: Characterization of Extracellular Vesicles
  • 05:35Conclusion

Summary

自动翻译

自动翻译

The proposed protocol includes guidelines on how to avoid contamination with endotoxin during the isolation of extracellular vesicles from cell culture supernatants, and how to properly evaluate them.

Tags

Extracellular VesiclesEndotoxinLow-endotoxinCell CultureSupernatantUltracentrifugationFiltrationSW480SW620PBSFBSAseptic ProcedurePyrogenicLow Protein Binding

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique

Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Read Article