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Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins
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Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

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10:07 min

March 17, 2023

DOI:

10:07 min
March 17, 2023

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Summary

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Capturing dynamic changes in the protein activation of enucleated red blood cells poses methodological challenges, like the preservation of dynamic changes to acute stimuli for later assessment. The presented protocol describes sample preparation and staining techniques that enable preservation and analysis of relevant protein changes and subsequent detection.

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