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Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

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10:07 min

March 17, 2023

DOI:

10:07 min
March 17, 2023

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Summary

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Capturing dynamic changes in the protein activation of enucleated red blood cells poses methodological challenges, like the preservation of dynamic changes to acute stimuli for later assessment. The presented protocol describes sample preparation and staining techniques that enable preservation and analysis of relevant protein changes and subsequent detection.

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