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Chapters
- 00:05Introduction
- 00:48Staining of RBC Proteins Using Immunohistochemistry
- 06:10Fluorescent Labelling of RBC Proteins
- 08:41Results: Detection of Dynamic Alternations in RBC‐NOS Phosphorylation
- 09:32Conclusion
Capturing dynamic changes in the protein activation of enucleated red blood cells poses methodological challenges, like the preservation of dynamic changes to acute stimuli for later assessment. The presented protocol describes sample preparation and staining techniques that enable preservation and analysis of relevant protein changes and subsequent detection.
