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Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging
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Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

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09:11 min

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April 28, 2023

DOI:

10.3791/64662-v

DOI:
10.3791/64662-v

•

09:11 min
April 28, 2023

•

33 Views
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1Department of Biological Sciences,University of Arkansas

Summary

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Here, a protocol for the implementation of expansion microscopy in early Drosophila embryos to achieve super-resolution imaging using a conventional laser-scanning confocal microscope is presented.

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Parveen, S., Jones, N. W., Millerschultz, I., Paré, A. C. Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging. J. Vis. Exp. (194), e64662, doi:10.3791/64662 (2023).

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