Here, a protocol for the implementation of expansion microscopy in early Drosophila embryos to achieve super-resolution imaging using a conventional laser-scanning confocal microscope is presented.
Parveen, S., Jones, N. W., Millerschultz, I., Paré, A. C. Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging. J. Vis. Exp. (194), e64662, doi:10.3791/64662 (2023).