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从小鼠中分离和培养骨髓来源的巨噬细胞
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Isolation and Culture of Bone Marrow-Derived Macrophages from Mice
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从小鼠中分离和培养骨髓来源的巨噬细胞

1,251 Views

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07:12 min

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June 23, 2023

DOI:

10.3791/64566-v

DOI:
10.3791/64566-v

•

07:12 min
June 23, 2023

•

53 Views
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1Department of General Pathology, Institute of Biological Sciences,Federal University of Minas Gerais, 2Department of Cell Biology and Molecular Genetics,University of Maryland

Summary

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本方案描述了从小鼠中分离和培养骨髓来源的巨噬细胞。

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Bone MarrowMacrophagesIsolationCultureMurineC57BL/6Sterile TechniqueCentrifugationCell SuspensionDMEM/F12

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Cite this Article

Gonçalves, R., Kaliff Teófilo Murta, G., Aparecida de Souza, I., Mosser, D. M. Isolation and Culture of Bone Marrow-Derived Macrophages from Mice. J. Vis. Exp. (196), e64566, doi:10.3791/64566 (2023).

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