Dergi
/
İmmünoloji ve Enfeksiyon
/
Isolation and Culture of Bone Marrow-Derived Macrophages from Mice
JoVE Journal
İmmünoloji ve Enfeksiyon
Author Produced
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

DOI:
10.3791/64566-v

•

07:12 min

•

June 23, 2023

•

, , ,
1Department of General Pathology, Institute of Biological Sciences,Federal University of Minas Gerais, 2Department of Cell Biology and Molecular Genetics,University of Maryland

Bölümler

  • 00:00Introduction
  • 01:08Removal of the Femur and Tibia
  • 01:44Isolation of Bone Marrow Progenitors from Tibia and Femur
  • 03:07Macrophage Culture
  • 03:57Harvesting Macrophages
  • 05:39Results: Characterizing the Bone Marrow-Derived Macrophages
  • 06:42Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The present protocol describes the isolation and culture of bone marrow-derived macrophages from mice.

Etiketler

Bone MarrowMacrophagesIsolationCultureMurineC57BL/6Sterile TechniqueCentrifugationCell SuspensionDMEM/F12

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Depletion and Reconstitution of Macrophages in Mice

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Bone Marrow-derived Macrophage Production

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Read Article