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Isolation and Culture of Bone Marrow-Derived Macrophages from Mice
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

1,455 Views

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07:12 min

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June 23, 2023

DOI:

10.3791/64566-v

DOI:
10.3791/64566-v

•

07:12 min
June 23, 2023

•

53 Views
, , ,
1Department of General Pathology, Institute of Biological Sciences,Federal University of Minas Gerais, 2Department of Cell Biology and Molecular Genetics,University of Maryland

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The present protocol describes the isolation and culture of bone marrow-derived macrophages from mice.

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Bone MarrowMacrophagesIsolationCultureMurineC57BL/6Sterile TechniqueCentrifugationCell SuspensionDMEM/F12

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Gonçalves, R., Kaliff Teófilo Murta, G., Aparecida de Souza, I., Mosser, D. M. Isolation and Culture of Bone Marrow-Derived Macrophages from Mice. J. Vis. Exp. (196), e64566, doi:10.3791/64566 (2023).

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