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下拉测定与细菌细胞共表达相结合，作为测试具有挑战性的蛋白质-蛋白质相互作用的省时工具

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Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

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下拉测定与细菌细胞共表达相结合，作为测试具有挑战性的蛋白质-蛋白质相互作用的省时工具

DOI:

10.3791/64541-v

•

07:03 min

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December 23, 2022

•

Artem Bonchuk², Nikolay Zolotarev, Konstantin Balagurov², Olga Arkova, Pavel Georgiev

¹Department of the Control of Genetic Processes, Institute of Gene Biology,Russian Academy of Sciences, ²Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology,Russian Academy of Sciences

Chapters

00:04Introduction
00:56Co-Transformation of Escherichia coli
02:18Expression
03:21Pulldown Assay
04:52Results: Bacterial Co-Expression of Differentially Tagged Proteins
06:15Conclusion

Summary

자동 번역

在这里，我们描述了一种使用一组兼容载体对差异标记蛋白质进行细菌共表达的方法，然后使用传统的下拉技术来研究无法 在体外组装的蛋白质复合物。

Tags

Pulldown Assay Protein-protein Interactions Co-expression Heterodimers Protein Complex Assembly Expression Optimization Rapid Screening Escherichia Coli Bacterial Transformation IPTG Induction Cell Lysis Affinity Purification

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