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A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro
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A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro

A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro

3,432 Views

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11:06 min

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October 06, 2022

DOI:

10.3791/64469-v

DOI:
10.3791/64469-v

•

11:06 min
October 06, 2022

•

8 Views
, , , , ,
1Department of Biochemistry and Molecular Biology, Miller School of Medicine,University of Miami, 2Institute of Physiotherapy and Health Sciences,The Jerzy Kukuczka Academy of Physical Education

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Here, we describe the method for establishing a triple cell culture model of the blood-brain barrier based on primary human brain microvascular endothelial cells, astrocytes, and pericytes. This multicellular model is suitable for studies of neurovascular unit dysfunction during ischemic stroke in vitro or for the screening of drug candidates.

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Blood-brain BarrierIschemic StrokeIn Vitro ModelTriple Cell CulturePericytesAstrocytesCell Culture ProtocolCell DensityTrypsinizationCell CountingCell Seeding

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Cite this Article

Fattakhov, N., Torices, S., Becker, S., Teglas, T., Naranjo, O., Toborek, M. A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro. J. Vis. Exp. (188), e64469, doi:10.3791/64469 (2022).

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