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A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro
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Biochemie
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JoVE Journal Biochemie
A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro

A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro

DOI:
10.3791/64469-v

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11:06 min

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October 06, 2022

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, , , , ,
1Department of Biochemistry and Molecular Biology, Miller School of Medicine,University of Miami, 2Institute of Physiotherapy and Health Sciences,The Jerzy Kukuczka Academy of Physical Education

Kapitel

  • 00:05Introduction
  • 00:50Triple Cell Culture BBB Model Setting
  • 03:35Induction of the Oxygen-Glucose Deprivation
  • 05:19TEER Measurements
  • 06:49Assessment of the BBB Paracellular Permeability
  • 07:40Detection of Hypoxia in Live Cells
  • 08:41Results: A Method to Set up an In Vitro Reliable Triple Endothelial Cell-Pericyte-Astrocyte Culture BBB Model
  • 10:20Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we describe the method for establishing a triple cell culture model of the blood-brain barrier based on primary human brain microvascular endothelial cells, astrocytes, and pericytes. This multicellular model is suitable for studies of neurovascular unit dysfunction during ischemic stroke in vitro or for the screening of drug candidates.

Tags

Blood-brain BarrierIschemic StrokeIn Vitro ModelTriple Cell CulturePericytesAstrocytesCell Culture ProtocolCell DensityTrypsinizationCell CountingCell Seeding

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