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神経炎症と神経毒性を評価するためのリポ多糖の仔魚への脳室マイクロインジェクション
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JoVE 신문 신경과학
Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity
DOI:

07:31 min

August 23, 2022

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Chapters

  • 00:05Introduction
  • 00:39Preparing and Mounting Zebrafish for Microinjections
  • 02:22Injecting the Brain Ventricle
  • 03:08Imaging
  • 03:43Determination of Gene Expression Markers
  • 04:44Zebrafish Locomotive Behavioral Assay
  • 05:34Results: Lipopolysaccharide-Induced Neuroinflammation to Assess the Levels of Proinflammatory Mediators
  • 07:02Conclusion

Summary

자동 번역

このプロトコルは、ゼブラフィッシュ幼虫モデルの脳室領域へのリポ多糖のマイクロインジェクションを実証し、結果として生じる神経炎症反応と神経毒性を研究します。

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