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Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity
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JoVE Journal Neurowissenschaften
Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

2,668 Views

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07:31 min

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August 23, 2022

DOI:

10.3791/64313-v

DOI:
10.3791/64313-v

•

07:31 min
August 23, 2022

•

18 Views
,
1State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences,University of Macau, Avenida da Universidade, 2Department of Pharmaceutical Sciences, Faculty of Health Sciences,University of Macau, Avenida da Universidade

Summary

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This protocol demonstrates the microinjection of lipopolysaccharide into the brain ventricular region in a zebrafish larval model to study the resulting neuroinflammatory response and neurotoxicity.

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Brain VentricleMicroinjectionLipopolysaccharideNeuroinflammationNeurotoxicityZebrafishIn Vivo ModelAnti-neuroinflammatory DrugsGlass CapillaryMicromanipulatorAgaroseAnesthesia

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Cite this Article

He, Y., Lee, S. M. Y. Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity. J. Vis. Exp. (186), e64313, doi:10.3791/64313 (2022).

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