Journal
/
신경과학
/
Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

DOI:
10.3791/64313-v

•

07:31 min

•

August 23, 2022

•

,
1State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences,University of Macau, Avenida da Universidade, 2Department of Pharmaceutical Sciences, Faculty of Health Sciences,University of Macau, Avenida da Universidade

Chapters

  • 00:05Introduction
  • 00:39Preparing and Mounting Zebrafish for Microinjections
  • 02:22Injecting the Brain Ventricle
  • 03:08Imaging
  • 03:43Determination of Gene Expression Markers
  • 04:44Zebrafish Locomotive Behavioral Assay
  • 05:34Results: Lipopolysaccharide-Induced Neuroinflammation to Assess the Levels of Proinflammatory Mediators
  • 07:02Conclusion

Summary

자동 번역

자동 번역

This protocol demonstrates the microinjection of lipopolysaccharide into the brain ventricular region in a zebrafish larval model to study the resulting neuroinflammatory response and neurotoxicity.

Tags

Brain VentricleMicroinjectionLipopolysaccharideNeuroinflammationNeurotoxicityZebrafishIn Vivo ModelAnti-neuroinflammatory DrugsGlass CapillaryMicromanipulatorAgaroseAnesthesia

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Targeting of Deep Brain Structures with Microinjections for Delivery of Drugs, Viral Vectors, or Cell Transplants

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

Manual Drainage of the Zebrafish Embryonic Brain Ventricles

Forebrain Electrophysiological Recording in Larval Zebrafish

Spinal Cord Transection in the Larval Zebrafish

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

Personalized Needles for Microinjections in the Rodent Brain

Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

Read Article