Journal
/
신경과학
/
Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage

Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage

1,624 Views

•

07:13 min

•

February 10, 2023

DOI:

10.3791/64286-v

DOI:
10.3791/64286-v

•

07:13 min
February 10, 2023

•

29 Views
, ,
1Department of Audiology and Speech-Language Pathology,MacKay Medical College, 2Institute of Biomedical Sciences,MacKay Medical College

Summary

Automatically generated

Here, we present a protocol for a mouse model of noise-induced hearing loss (NIHL). To induce NIHL, we developed a new and simple device using corrugated plastic, a rat trap cage, and a speaker. Auditory brainstem response and immunofluorescence imaging were employed to assess the hearing function and outer hair cell damage, respectively.

Explore More Videos

Noise-induced Hearing LossMice ModelAuditory Brainstem Response (ABR)Experimental ProcedureHearing ThresholdsOuter Hair Cell DamageSound ExposureC57BL/6J MiceSoundproof BoxNoise CalibrationDecibels SPL-AFrequency SettingsCage PreparationTreatment Strategies

Read Article

Cite this Article

Lee, D., Pan, Y., Hsu, Y. Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage. J. Vis. Exp. (192), e64286, doi:10.3791/64286 (2023).

Download .ris file

Copy

Share Video

.

Copy