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Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage
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JoVE Journal Nörobilim
Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage

Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage

1,624 Views

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07:13 min

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February 10, 2023

DOI:

10.3791/64286-v

DOI:
10.3791/64286-v

•

07:13 min
February 10, 2023

•

29 Views
, ,
1Department of Audiology and Speech-Language Pathology,MacKay Medical College, 2Institute of Biomedical Sciences,MacKay Medical College

Özet

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Here, we present a protocol for a mouse model of noise-induced hearing loss (NIHL). To induce NIHL, we developed a new and simple device using corrugated plastic, a rat trap cage, and a speaker. Auditory brainstem response and immunofluorescence imaging were employed to assess the hearing function and outer hair cell damage, respectively.

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Lee, D., Pan, Y., Hsu, Y. Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage. J. Vis. Exp. (192), e64286, doi:10.3791/64286 (2023).

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