Journal
/
/
C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining
JoVE 신문
유전학
This content is Free Access.
JoVE 신문 유전학
C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

4,334 Views

06:04 min

September 16, 2022

DOI:

06:04 min
September 16, 2022

13 Views
, , ,

Summary

Automatically generated

This is a commonly used method for C. elegans gonad dissection followed by freeze crack, which produces germline samples for immunofluorescence via antibody staining, or for simple DAPI staining to visualize DNA. This protocol has been successful for undergraduates in a research lab and in a course-based undergraduate research experience.

Read Article