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JoVE Journal Genetics
C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining
 

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

Article DOI: 10.3791/64204-v • 06:04 min • September 16th, 2022
September 16th, 2022 •
*1, *1, 1, 1
1Department of Biological Sciences, University of Massachusetts Lowell
* These authors contributed equally

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This is a commonly used method for C. elegans gonad dissection followed by freeze crack, which produces germline samples for immunofluorescence via antibody staining, or for simple DAPI staining to visualize DNA. This protocol has been successful for undergraduates in a research lab and in a course-based undergraduate research experience.

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C. Elegans Gonad Dissection Freeze Crack Immunofluorescence DAPI Staining Antibody Tagged Fusion Protein Dissection Technique Germline Extrusions Holding Slide Coverslip Dissecting Solution Hermaphrodites NGM Plate Needles X Shape Pinning Down Pharynx Decapitate
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