Journal
/
생화학
/
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

2,087 Views

•

06:32 min

•

July 28, 2022

DOI:

10.3791/64090-v

DOI:
10.3791/64090-v

•

06:32 min
July 28, 2022

•

2 Views
, , ,
1Department of Biochemistry and Biophysics,University of North Carolina at Chapel Hill, 2Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, 3Department of Biology,University of North Carolina at Chapel Hill

Summary

Automatically generated

Cell-free reconstitution has been a key tool to understand the cytoskeleton assembly, and work in the last decade has established approaches to study septin dynamics in minimal systems. Presented here are three complementary methods to observe septin assembly in different membrane contexts: planar bilayers, spherical supports, and rod supports.

Explore More Videos

Membrane BindingMembrane ShapeProtein AssemblyLipid BilayerLiposomesPlasma CleaningUV-activated AdhesiveSupported Lipid BilayerSeptinsTIRF MicroscopySilica MicrospheresSUVs

Read Article

Cite this Article

Curtis, B. N., Vogt, E. J. D., Cannon, K. S., Gladfelter, A. S. Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions. J. Vis. Exp. (185), e64090, doi:10.3791/64090 (2022).

Download .ris file

Copy

Share Video

.

Copy