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Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

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06:32 min

July 28, 2022

DOI:

06:32 min
July 28, 2022

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Summary

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Cell-free reconstitution has been a key tool to understand the cytoskeleton assembly, and work in the last decade has established approaches to study septin dynamics in minimal systems. Presented here are three complementary methods to observe septin assembly in different membrane contexts: planar bilayers, spherical supports, and rod supports.

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