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Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
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Bioquímica
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JoVE Journal Bioquímica
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

DOI:
10.3791/64090-v

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06:32 min

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July 28, 2022

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, , ,
1Department of Biochemistry and Biophysics,University of North Carolina at Chapel Hill, 2Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, 3Department of Biology,University of North Carolina at Chapel Hill

Capítulos

  • 00:05Introduction
  • 00:42Planar Lipid Bilayers
  • 02:46Spherical Supported Lipid Bilayers
  • 04:50Results: Micrographs of Planar Bilayers and Lipid-Coated Microspheres
  • 06:00Conclusion

Summary

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Cell-free reconstitution has been a key tool to understand the cytoskeleton assembly, and work in the last decade has established approaches to study septin dynamics in minimal systems. Presented here are three complementary methods to observe septin assembly in different membrane contexts: planar bilayers, spherical supports, and rod supports.

Tags

Membrane BindingMembrane ShapeProtein AssemblyLipid BilayerLiposomesPlasma CleaningUV-activated AdhesiveSupported Lipid BilayerSeptinsTIRF MicroscopySilica MicrospheresSUVs

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