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毫秒氢/氘交换质谱法用于生理条件下α-突触核蛋白结构动力学的研究

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Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions

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毫秒氢/氘交换质谱法用于生理条件下α-突触核蛋白结构动力学的研究

2,603 Views

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08:40 min

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June 23, 2022

DOI:

10.3791/64050-v

DOI:

10.3791/64050-v

•

08:40 min

June 23, 2022

•

8 Views

Neeleema Seetaloo¹, Jonathan J. Phillips¹

¹Living Systems Institute, Department of Biosciences,University of Exeter

Summary

单体α-突触核蛋白的结构集合影响其生理功能和理化性质。本方案描述了如何进行毫秒氢/氘交换质谱和随后的数据分析，以确定在生理条件下这种内在无序蛋白质的单体的构象信息。

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