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Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions
 

Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions

Article DOI: 10.3791/64050-v • 08:40 min • June 23rd, 2022
June 23rd, 2022 •
1, 1
1Living Systems Institute, Department of Biosciences, University of Exeter

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Resumo
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The structural ensemble of monomeric alpha-synuclein affects its physiological function and physicochemical properties. The present protocol describes how to perform millisecond hydrogen/deuterium-exchange mass spectrometry and subsequent data analyses to determine conformational information on the monomer of this intrinsically disordered protein under physiological conditions.

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Keywords: Alpha-synuclein Hydrogen/deuterium Exchange Mass Spectrometry Intrinsically Disordered Proteins Peptide Mapping Sample Preparation Autosampler HDX Robotics Quench Buffer Peptide Coverage Map Fast HDX Prototype Instrument HDX Data Analysis Software
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