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면역학 및 감염병학

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用于测试新兴抗生素的新型高通量离体绵羊皮肤伤口模型

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics

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用于测试新兴抗生素的新型高通量离体绵羊皮肤伤口模型

DOI:

10.3791/64041-v

•

08:30 min

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September 16, 2022

•

Hannah C. Regan², Annette F. Taylor², Esther Karunakaran²

¹Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB),University of Sheffield, ²Department of Chemical and Biological Engineering,University of Sheffield

Chapters

00:05Introduction
00:53Sterilization and Preparation of the Head
03:06Maintenance of Skin Samples
04:18Infection of Skin Samples
05:27Determination of the Bacterial Load
06:32Results: An Ex Vivo Ovine Wounded Skin Model Infected with Staphylococcus aureus
07:42Conclusion

Summary

자동 번역

该协议描述了一种逐步建立感染金黄色葡萄球菌的离体绵羊受伤皮肤模型的方法。与传统微生物学技术相比，这种高通量模型更好地模拟体内感染，并为研究人员提供了一个生理相关的平台来测试新兴抗菌剂的功效。

Tags

Ovine Skin Wound Model High-throughput Ex Vivo Antimicrobial Efficacy Topical Antimicrobials Wound Infections Contamination Aseptic Technique Skin Sample Chlorine Dioxide Solution Sterile PBS Air-liquid Interface

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