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鸡颅神经嵴细胞培养物的制备及形态学分析
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Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures
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鸡颅神经嵴细胞培养物的制备及形态学分析

2,219 Views

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09:26 min

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June 27, 2022

DOI:

10.3791/63799-v

DOI:
10.3791/63799-v

•

09:26 min
June 27, 2022

•

9 Views
, , ,
1Department of Biology and Neuroscience Program,Hamline University, 2Department of Genetics, Cell Biology and Development,University of Minnesota, 3Developmental Biology Center,University of Minnesota

Summary

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这种多功能方案描述了通过从鸡胚胎中切除颅神经褶皱来分离前迁移神经嵴细胞（NCC）。在铺板和孵育时，迁移性NCC从神经折叠外植体中出现，允许在简化的2D环境中评估细胞形态和迁移。

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Cite this Article

Jacques-Fricke, B. T., Roffers-Agarwal, J., Gustafson, C. M., Gammill, L. S. Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures. J. Vis. Exp. (184), e63799, doi:10.3791/63799 (2022).

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