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Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
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Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

1,587 Views

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08:48 min

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April 21, 2022

DOI:

10.3791/63790-v

DOI:
10.3791/63790-v

•

08:48 min
April 21, 2022

•

14 Views
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1Institute of Toxicology, College of Preventive Medicine,Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine,Army Medical University (Third Military Medical University)

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The present protocol provides a practical solution that allows for the measurement of apoptosis, mitochondrial membrane potential, and DNA damage in human sperm with a single cytometer.

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Ling, X., Zou, P., Ao, L., Zhou, N., Wang, X., Sun, L., Yang, H., Liu, J., Cao, J., Chen, Q. Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects. J. Vis. Exp. (182), e63790, doi:10.3791/63790 (2022).

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