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Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
JoVE Journal
Medicina
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JoVE Journal Medicina
Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

1,587 Views

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08:48 min

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April 21, 2022

DOI:

10.3791/63790-v

DOI:
10.3791/63790-v

•

08:48 min
April 21, 2022

•

14 Views
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1Institute of Toxicology, College of Preventive Medicine,Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine,Army Medical University (Third Military Medical University)

Summary

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The present protocol provides a practical solution that allows for the measurement of apoptosis, mitochondrial membrane potential, and DNA damage in human sperm with a single cytometer.

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Cite this Article

Ling, X., Zou, P., Ao, L., Zhou, N., Wang, X., Sun, L., Yang, H., Liu, J., Cao, J., Chen, Q. Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects. J. Vis. Exp. (182), e63790, doi:10.3791/63790 (2022).

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