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Beoordeling van mitochondriale functie in heupzenuw door middel van hoge resolutie respirometrie
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Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry
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Beoordeling van mitochondriale functie in heupzenuw door middel van hoge resolutie respirometrie

2,117 Views

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08:19 min

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May 05, 2022

DOI:

10.3791/63690-v

DOI:
10.3791/63690-v

•

08:19 min
May 05, 2022

•

11 Views
,
1Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde,Universidade Federal do Rio de Janeiro

Summary

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Hoge resolutie respirometrie gekoppeld aan fluorescentiesensoren bepaalt mitochondriaal zuurstofverbruik en reactieve zuurstofsoorten (ROS) generatie. Het huidige protocol beschrijft een techniek om mitochondriale ademhalingssnelheden en ROS-productie in de gepermeabiliseerde heupzenuw te beoordelen.

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Formiga-Jr, M. A., Camacho-Pereira, J. Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry. J. Vis. Exp. (183), e63690, doi:10.3791/63690 (2022).

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