/
/
Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Introduction
- 01:19Calibration of Polarographic Oxygen Sensors for High-Resolution Respirometry (HRR)
- 01:56Dissection and Permeabilization of the Sciatic Nerve
- 02:58Oxygen Consumption and Reactive Oxygen Species (ROS) Production Determination
- 05:51Results: Assessing the Mitochondrial Oxygen Consumption by the Permeabilized Sciatic Nerve
- 07:41Conclusion
High-resolution respirometry coupled to fluorescence sensors determines mitochondrial oxygen consumption and reactive oxygen species (ROS) generation. The present protocol describes a technique to assess mitochondrial respiratory rates and ROS production in the permeabilized sciatic nerve.
