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Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry
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Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry
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May 05, 2022
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High-resolution respirometry coupled to fluorescence sensors determines mitochondrial oxygen consumption and reactive oxygen species (ROS) generation. The present protocol describes a technique to assess mitochondrial respiratory rates and ROS production in the permeabilized sciatic nerve.
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Formiga-Jr, M. A., Camacho-Pereira, J. Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry. J. Vis. Exp. (183), e63690, doi:10.3791/63690 (2022).
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