JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
2,879 Views
•
09:30 min
•
August 17, 2022
DOI:
1Centre for Women, Children, and Reproduction,Guangxi Key Laboratory of Metabolic Diseases Research, 2Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology,Southern Medical University, 3Department of Obstetrics and Gynecology,Chinese PLA General Hospital, 4Clinical Laboratory,Northern Theater Air Force Hospital, 5Guangzhou Darui Reproduction Technology Co., Ltd.
Summary
Automatically generated
The protocol presents the overall in-lab procedures required in pre-implantation genetic testing for aneuploidy on a semiconductor-based next-generation sequencing platform. Here we present the detailed steps of whole genome amplification, DNA fragment selection, library construction, template preparation, and sequencing working flow with representative results.
Read Article
Cite this Article
Xu, C., Wei, R., Lin, H., Deng, L., Wang, L., Li, D., Den, H., Qin, W., Wen, P., Liu, Y., Wu, Y., Ma, Q., Duan, J. Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform. J. Vis. Exp. (186), e63493, doi:10.3791/63493 (2022).
Copy