JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Singola cellula riutilizzabile per analisi epigenomiche iterative
1Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute,National Institutes of Health, 2Biostatistics and Data Management Section, Center for Cancer Research, National Cancer Institute,National Institutes of Health, 3CCR Collaborative Bioinformatics Resource (CCBR), Center for Cancer Research, National Cancer Institute,National Institutes of Health, 4Advanced Biomedical Computational Science,Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute
Chapters
- 00:05Introduction
- 01:32Preparation of Reusable Single Cells: Manual Version
- 03:09Random Primer Annealing and Extension
- 04:09Antibody Binding and Proximity Ligation of Antibody Probe and Proximally Extended Random Primer
- 05:25Full Extension of the 1st Random Primer and Multiple Displacement Amplification
- 07:32Results: Single Cell Multiepigenomic Analysis
- 09:26Conclusion
Il presente protocollo descrive un metodo a singola cellula per analisi epigenomiche iterative utilizzando una singola cellula riutilizzabile. La singola cellula riutilizzabile consente l'analisi di più segni epigenetici nella stessa singola cellula e la convalida statistica dei risultati.
