Journal
/
Génétique
/
Reusable Single Cell for Iterative Epigenomic Analyses
Journal JoVE
Génétique
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Génétique
Reusable Single Cell for Iterative Epigenomic Analyses

Reusable Single Cell for Iterative Epigenomic Analyses

DOI:
10.3791/63456-v

•

10:28 min

•

February 11, 2022

•

, , ,
1Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute,National Institutes of Health, 2Biostatistics and Data Management Section, Center for Cancer Research, National Cancer Institute,National Institutes of Health, 3CCR Collaborative Bioinformatics Resource (CCBR), Center for Cancer Research, National Cancer Institute,National Institutes of Health, 4Advanced Biomedical Computational Science,Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute

Chapitres

  • 00:05Introduction
  • 01:32Preparation of Reusable Single Cells: Manual Version
  • 03:09Random Primer Annealing and Extension
  • 04:09Antibody Binding and Proximity Ligation of Antibody Probe and Proximally Extended Random Primer
  • 05:25Full Extension of the 1st Random Primer and Multiple Displacement Amplification
  • 07:32Results: Single Cell Multiepigenomic Analysis
  • 09:26Conclusion

Summary

Traduction automatique

Traduction automatique

The present protocol describes a single-cell method for iterative epigenomic analyses using a reusable single cell. The reusable single cell allows analyses of multiple epigenetic marks in the same single cell and statistical validation of the results.

Tags

Reusable Single CellEpigenomic AnalysisSingle-cell EpigenomeEpigenetic TherapyEpigenome RegulationGene ExpressionIntercalator DyeEDTA PBSPCR TubeSwing RotorLiquid Handling RobotTEMEDMineral OilTP Magnesium Negative BufferAnnealing Buffer

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Detection of Copy Number Alterations Using Single Cell Sequencing

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Read Article