Here we describe two modifications of the DNA fiber assay to investigate single-stranded DNA gaps in replicating DNA after lesion induction. The S1 fiber assay enables the detection of post-replicative gaps using the ssDNA-specific S1 endonuclease, while the gap-filling assay allows visualization and quantification of gap repair.
Martins, D. J., Tirman, S., Quinet, A., Menck, C. F. M. Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay. J. Vis. Exp. (180), e63448, doi:10.3791/63448 (2022).