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JoVE Journal Biochemistry
通过TIRF显微镜 在体外 同时可视化交联和单微管的动力学
 
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通过TIRF显微镜 在体外 同时可视化交联和单微管的动力学

Article DOI: 10.3791/63377-v • 07:20 min • February 18th, 2022
February 18th, 2022 •
*1,2, *1, 1,2
1Molecular Biology, Massachusetts General Hospital, 2Department of Genetics, Harvard Medical School
* These authors contributed equally

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在这里，提出了基于TIRF显微镜 的体外 重建测定，以同时量化和比较两个微管群体的动力学。描述了一种同时观察多个微管相关蛋白在交联微管束和单个微管上的集体活性的方法。

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《生物化学》第180期，
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