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Kvantitativ mikrotubulusfraktioneringsteknik til adskillelse af stabile mikrotubuli, labile mikrotubuli og frit tubulin i musevæv
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Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Kvantitativ mikrotubulusfraktioneringsteknik til adskillelse af stabile mikrotubuli, labile mikrotubuli og frit tubulin i musevæv

1,548 Views

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07:21 min

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November 17, 2023

DOI:

10.3791/63358-v

DOI:
10.3791/63358-v

•

07:21 min
November 17, 2023

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24 Views
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1Department of Neuropathology, Faculty of Life and Medical Sciences,Doshisha University, 2Center for Research in Neurodegenerative Diseases,Doshisha University, 3Laboratory of Physiology and Anatomy, School of Pharmacy,Nihon University

Summary

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Mikrotubuli, som er tubulinpolymerer, spiller en afgørende rolle som cytoskeletkomponent i eukaryote celler og er kendt for deres dynamiske ustabilitet. Denne undersøgelse udviklede en metode til fraktionering af mikrotubuli for at adskille dem i stabile mikrotubuli, labile mikrotubuli og frit tubulin for at evaluere stabiliteten af mikrotubuli i forskellige musevæv.

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Keywords Extracted From The Given Text:Quantitative Microtubule FractionationStable MicrotubulesLabile MicrotubulesFree TubulinMicrotubule StabilityAlzheimer’s DiseaseCancerPhosphate Buffer SolutionPBSMicrotubule Stabilizing BufferMSBCentrifugationSupernatantPrecipitateSodium Dodecyl SulfateSDS Sample BufferWestern Blotting

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Hagita-Tatsumoto, A., Miyasaka, T. Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues. J. Vis. Exp. (201), e63358, doi:10.3791/63358 (2023).

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