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Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
Chapters
- 00:05Introduction
- 00:49Isolation and Homogenization of Mouse Tissues to Quantify Microtubule Fractions
- 01:53Fractionation of Mouse Tissues
- 04:04Results: Analysis of the Characterized Murine Microtubule Brain Fractions
- 06:25Conclusion
Microtubules, which are tubulin polymers, play a crucial role as a cytoskeleton component in eukaryotic cells and are known for their dynamic instability. This study developed a method for fractionating microtubules to separate them into stable microtubules, labile microtubules, and free tubulin to evaluate the stability of microtubules in various mouse tissues.
Tags
Keywords Extracted From The Given Text:Quantitative Microtubule FractionationStable MicrotubulesLabile MicrotubulesFree TubulinMicrotubule StabilityAlzheimer’s DiseaseCancerPhosphate Buffer SolutionPBSMicrotubule Stabilizing BufferMSBCentrifugationSupernatantPrecipitateSodium Dodecyl SulfateSDS Sample BufferWestern Blotting
