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Biochemistry

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JoVE Journal Biochemistry
Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
 

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Article DOI: 10.3791/63358-v • 07:21 min • November 17th, 2023
November 17th, 2023 •
1,2, 1,2,3
1Department of Neuropathology, Faculty of Life and Medical Sciences, Doshisha University, 2Center for Research in Neurodegenerative Diseases, Doshisha University, 3Laboratory of Physiology and Anatomy, School of Pharmacy, Nihon University

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요약
Automatic Translation

Microtubules, which are tubulin polymers, play a crucial role as a cytoskeleton component in eukaryotic cells and are known for their dynamic instability. This study developed a method for fractionating microtubules to separate them into stable microtubules, labile microtubules, and free tubulin to evaluate the stability of microtubules in various mouse tissues.

Tags

Tubulin labile microtubule stable microtubule post-translational modification microtubule-associated protein fractionation microtubule-targeting agents
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