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6-羟基多巴胺诱导的基于斑马鱼的成人帕金森病模型的运动评估

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Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model

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6-羟基多巴胺诱导的基于斑马鱼的成人帕金森病模型的运动评估

DOI:

10.3791/63355-v

•

07:32 min

•

December 28, 2021

•

Naemah Md Hamzah, Siong Meng Lim, Yuganthini Vijayanathan², Fei Tieng Lim, Abu Bakar Abdul Majeed, Maw Pin Tan, Kalavathy Ramasamy

¹Collaborative Drug Discovery Research (CDDR) Group and Brain Degeneration and Therapeutics Group, Faculty of Pharmacy,Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, ²Department of Medicine, Faculty of Medicine,Universiti Malaya

Chapters

00:05Introduction
01:00Zebrafish Maintenance and Pre-ICV Microinjection Preparations
02:19Anaesthetisation and ICV Injection of Zebrafish
04:10Locomotor Assessment
05:22Results: Assessment of Neuroregeneration in Zebrafish Based PD Model
06:44Conclusion

Summary

자동 번역

本方案描述了在腹侧间脑（Dn）处用神经毒性6-羟基多巴胺（6-OHDA）向成年斑马鱼注射脑室（ICV）以及通过使用开放水箱测试评估损伤和随后恢复的游泳行为，并伴有使用视频跟踪软件进行分析。

Tags

Keywords: Zebrafish Parkinson’s Disease 6-hydroxydopamine Locomotor Assessment Motor Impairment Dopaminergic Neurons Intracerebroventricular Injection Neuroregeneration Cell Replacement Tricaine Methanesulfonate Anesthesia Microcapillary Injector

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