Journal
/
Neurociência
/
Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model

Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model

2,800 Views

•

07:32 min

•

December 28, 2021

DOI:

10.3791/63355-v

DOI:
10.3791/63355-v

•

07:32 min
December 28, 2021

•

28 Views
, , , , , ,
1Collaborative Drug Discovery Research (CDDR) Group and Brain Degeneration and Therapeutics Group, Faculty of Pharmacy,Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, 2Department of Medicine, Faculty of Medicine,Universiti Malaya

Summary

Automatically generated

The present protocol describes the intracerebroventricular (ICV) injection of adult zebrafish with neurotoxic 6-hydroxydopamine (6-OHDA) at the ventral diencephalon (Dn) and the assessment of the impairment and subsequent recovery of swimming behavior postlesion by using the open tank test, which is accompanied by analysis using a video tracking software.

Explore More Videos

ZebrafishParkinson’s Disease6-hydroxydopamineLocomotor AssessmentMotor ImpairmentDopaminergic NeuronsIntracerebroventricular InjectionNeuroregenerationCell ReplacementTricaine MethanesulfonateAnesthesiaMicrocapillary Injector

Read Article

Cite this Article

Md Hamzah, N., Lim, S. M., Vijayanathan, Y., Lim, F. T., Abdul Majeed, A. B., Tan, M. P., Ramasamy, K. Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson's Disease Model. J. Vis. Exp. (178), e63355, doi:10.3791/63355 (2021).

Download .ris file

Copy

Share Video

.

Copy