Rivista
/
Neuroscienze
/
Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model

Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model

DOI:
10.3791/63355-v

•

07:32 min

•

December 28, 2021

•

, , , , , ,
1Collaborative Drug Discovery Research (CDDR) Group and Brain Degeneration and Therapeutics Group, Faculty of Pharmacy,Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, 2Department of Medicine, Faculty of Medicine,Universiti Malaya

Capitoli

  • 00:05Introduction
  • 01:00Zebrafish Maintenance and Pre-ICV Microinjection Preparations
  • 02:19Anaesthetisation and ICV Injection of Zebrafish
  • 04:10Locomotor Assessment
  • 05:22Results: Assessment of Neuroregeneration in Zebrafish Based PD Model
  • 06:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

The present protocol describes the intracerebroventricular (ICV) injection of adult zebrafish with neurotoxic 6-hydroxydopamine (6-OHDA) at the ventral diencephalon (Dn) and the assessment of the impairment and subsequent recovery of swimming behavior postlesion by using the open tank test, which is accompanied by analysis using a video tracking software.

Tags

ZebrafishParkinson’s Disease6-hydroxydopamineLocomotor AssessmentMotor ImpairmentDopaminergic NeuronsIntracerebroventricular InjectionNeuroregenerationCell ReplacementTricaine MethanesulfonateAnesthesiaMicrocapillary Injector

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Methods to Characterize Spontaneous and Startle-induced Locomotion in a Rotenone-induced Parkinson’s Disease Model of Drosophila

Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease

Thoracic Spinal Cord Hemisection Surgery and Open-Field Locomotor Assessment in the Rat

Assessment of Long-term Depression Induction in Adult Cerebellar Slices

Applying the RatWalker System for Gait Analysis in a Genetic Rat Model of Parkinson’s Disease

The 6-hydroxydopamine Rat Model of Parkinson’s Disease

Induction and Assessment of Levodopa-induced Dyskinesias in a Rat Model of Parkinson’s Disease

Assessment of Swim Endurance and Swim Behavior in Adult Zebrafish

Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein

Author Spotlight: Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury

Read Article