Journal
/
신경과학
/
Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model

Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model

DOI:
10.3791/63355-v

•

07:32 min

•

December 28, 2021

•

, , , , , ,
1Collaborative Drug Discovery Research (CDDR) Group and Brain Degeneration and Therapeutics Group, Faculty of Pharmacy,Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, 2Department of Medicine, Faculty of Medicine,Universiti Malaya

Chapters

  • 00:05Introduction
  • 01:00Zebrafish Maintenance and Pre-ICV Microinjection Preparations
  • 02:19Anaesthetisation and ICV Injection of Zebrafish
  • 04:10Locomotor Assessment
  • 05:22Results: Assessment of Neuroregeneration in Zebrafish Based PD Model
  • 06:44Conclusion

Summary

자동 번역

자동 번역

The present protocol describes the intracerebroventricular (ICV) injection of adult zebrafish with neurotoxic 6-hydroxydopamine (6-OHDA) at the ventral diencephalon (Dn) and the assessment of the impairment and subsequent recovery of swimming behavior postlesion by using the open tank test, which is accompanied by analysis using a video tracking software.

Tags

Keywords: ZebrafishParkinson’s Disease6-hydroxydopamineLocomotor AssessmentMotor ImpairmentDopaminergic NeuronsIntracerebroventricular InjectionNeuroregenerationCell ReplacementTricaine MethanesulfonateAnesthesiaMicrocapillary Injector

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/63355?article_type=v

Methods to Characterize Spontaneous and Startle-induced Locomotion in a Rotenone-induced Parkinson’s Disease Model of Drosophila
check_url/kr/63355?article_type=v

Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease
check_url/kr/63355?article_type=v

Thoracic Spinal Cord Hemisection Surgery and Open-Field Locomotor Assessment in the Rat
check_url/kr/63355?article_type=v

Characterizing the Relationship Between Eye Movement Parameters and Cognitive Functions in Non-demented Parkinson’s Disease Patients with Eye Tracking
check_url/kr/63355?article_type=v

Applying the RatWalker System for Gait Analysis in a Genetic Rat Model of Parkinson’s Disease
check_url/kr/63355?article_type=v

The 6-hydroxydopamine Rat Model of Parkinson’s Disease
check_url/kr/63355?article_type=v

Induction and Assessment of Levodopa-induced Dyskinesias in a Rat Model of Parkinson’s Disease
check_url/kr/63355?article_type=v

Assessment of Swim Endurance and Swim Behavior in Adult Zebrafish
check_url/kr/63355?article_type=v

Analyzing the Parkinson’s Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein
check_url/kr/63355?article_type=v

Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury (Video) | JoVE

Read Article