Journal
/
생화학
/
离体 小鼠通过门静脉进行肝灌注
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Ex Vivo Hepatic Perfusion Through the Portal Vein in Mouse
Please note that all translations are automatically generated. Click here for the English version.

离体 小鼠通过门静脉进行肝灌注

DOI:
10.3791/63154-v

•

05:30 min

•

March 09, 2022

•

, ,
1Department of Biochemistry and Molecular Biology,University of Florida

Chapters

  • 00:04Introduction
  • 01:01Celiotomy
  • 01:44Cannulation of Portal Vein
  • 02:50Resection of Liver Post Portal Vein Cannulation
  • 04:18Results: Liver Function Assessment by Oxygen Consumption and Flow Rate Measurement
  • 04:45Conclusion

Summary

자동 번역

자동 번역

该协议描述了一种通过门静脉灌注切除完整小鼠肝脏以进行代谢研究的简单方法。

Tags

Ex VivoHepatic PerfusionPortal VeinMouseLiver MetabolismDisease ModelsNAFLDNASHPharmaceutical AssessmentAnesthesiaCeliotomyPortal Vein CannulationCatheterSutureSyringe

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

的pH依赖性活性的检测<em>大肠杆菌</em>陪护HdeB<em>体外</em>和<em>在体内</em

脑膜分离法<em>体检</em评估亚突触蛋白定位的方法

小鼠肝灌注肝细胞和正弦内皮细胞的纯化

测定植物铁含量的比色法

在利什曼原虫、人体细胞和小鼠睾丸中的 Polysome 分析

Langendorff 灌注后离体小鼠心脏 Polysomes 的动态蛋白质组学及 miRNA 分析

全飞头段耗氧率的测定与解释

用于筛查绝经后心血管功能障碍的外源雌激素治疗的活体雌激素缺乏小鼠模型

翻译核糖体亲性纯化(TRAP),用于从体内内皮细胞中分离RNA

在Vivo羟基基蛋白足迹研究卡诺哈布迪特人的蛋白质相互作用

Read Article