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Ex Vivo Hepatic Perfusion Through the Portal Vein in Mouse
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JoVE Journal Biochimica
Ex Vivo Hepatic Perfusion Through the Portal Vein in Mouse

Ex Vivo Hepatic Perfusion Through the Portal Vein in Mouse

DOI:
10.3791/63154-v

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05:30 min

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March 09, 2022

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, ,
1Department of Biochemistry and Molecular Biology,University of Florida

Capitoli

  • 00:04Introduction
  • 01:01Celiotomy
  • 01:44Cannulation of Portal Vein
  • 02:50Resection of Liver Post Portal Vein Cannulation
  • 04:18Results: Liver Function Assessment by Oxygen Consumption and Flow Rate Measurement
  • 04:45Conclusion

Summary

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The protocol describes a straightforward method of resectioning an intact mouse liver for metabolic studies through portal vein perfusion.

Tags

Ex VivoHepatic PerfusionPortal VeinMouseLiver MetabolismDisease ModelsNAFLDNASHPharmaceutical AssessmentAnesthesiaCeliotomyPortal Vein CannulationCatheterSutureSyringe

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