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Probenvorbereitung und relative Quantifizierung mittels reduktiver Methylierung von Aminen für Peptidomik-Studien
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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
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Probenvorbereitung und relative Quantifizierung mittels reduktiver Methylierung von Aminen für Peptidomik-Studien

2,093 Views

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08:00 min

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November 04, 2021

DOI:

10.3791/62971-v

DOI:
10.3791/62971-v

•

08:00 min
November 04, 2021

•

10 Views
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1Department of Biological and Environmental Sciences, Bioscience Institute,Sao Paulo State University (UNESP), 2Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute,Sao Paulo State University (UNESP)

Summary

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Dieser Artikel beschreibt eine Probenvorbereitungsmethode, die auf Wärmeinaktivierung basiert, um endogene Peptide zu erhalten und den Abbau post mortem zu vermeiden, gefolgt von einer relativen Quantifizierung mittels Isotopenmarkierung plus LC-MS.

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Cite this Article

Correa, C. N., Fiametti, L. O., Mazzi Esquinca, M. E., Castro, L. M. d. Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies. J. Vis. Exp. (177), e62971, doi:10.3791/62971 (2021).

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