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Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos
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Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos

Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos

2,323 Views

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06:57 min

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July 21, 2021

DOI:

10.3791/62782-v

DOI:
10.3791/62782-v

•

06:57 min
July 21, 2021

•

1 Views
,
1Department of Neurobiology,University of Utah, 2Departments of Neurobiology and Department of Internal Medicine, Division of Hematology and Hematologic Malignancies,University of Utah, School of Medicine

Summary

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When Transforming Growth Factor ß family precursor proteins are ectopically expressed in Xenopus laevis embryos, they dimerize, get cleaved and are secreted into the blastocoele, which begins at the late blastula to early gastrula stage. We describe a method for aspirating cleavage products from the blastocoele cavity for immunoblot analysis.

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Cite this Article

Kim, H., Christian, J. L. Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos. J. Vis. Exp. (173), e62782, doi:10.3791/62782 (2021).

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